ALKALINE PHOSPHATASE p-NPP, 125 mL

PRINCIPLE

Determination of p-Nitrophenol produced by the interaction of Alkaline Phosphatase with the substrate p – Nitrophenylphosphate allows the measurement of enzymatic activity. In optimum conditions the ΔAbs/min value is directly related to the Alkaline Phosphatase concentraction of the sample.

DIAGNOSTIC USE

Alkaline phosphatase is an enzyme whose concentration in serum may be altered by various diseases.

Its main clinical application is related to cases of obstructive liver disease, with signifi cant elevations of basal level, especially in extrahepatic obstruction.

In metabolic bone diseases the increase of alkaline phosphatase level is associated to increased osteoblastic activity.

In bone metastasis the increase appears only in the case of osteosclerotic alterations.

Single test result can not be used to make a clinical diagnosis. It should integrate clinical and laboratory data.