CREATININE, 2 x 100 mL

PRINCIPLE

In alkaline medium, creatinine with picric acid form a coloured compound, alkaline creatinine picrate, which is determined photometrically. The colour produced in the reaction is proportional to the concentration of creatinine in the sample under optimal assay conditions.

DIAGNOSTIC USE

Creatinine in serum increases in case of renal failure, acute or chronic, hyperthyroidism, acromegaly and gigantism. Increasing creatinine in urine occurs in diabetes mellitus, and infection. Also, exercise promotes increased excretion in the urine. During pregnancy or decreased muscle mass, serum creatinine concentration is reduced, while creatinine in urine is decreased in renal failure, myopathy, anemia or hypothyroidism. One single test result could not be used to make a clinical diagnosis. It should integrate clinical and laboratory data.