For in vitro determination of gamma-GT in serum or plasma.
State | Liquid |
Storage temp. | +2 / +8 ºC |
Technique | Modified Szasz method |
PRINCIPLE
Serum γ-glutamyl transferase (γ-GT) transfer the glutamiy group to cosubstrate glycyl-glycyne producing 5-amino-2-nitrobenzoate. In optimum conditions enzymatc activity is directly related to amino-2-nitrobenzoate produced that is measured by spectrophotometry
DIAGNOSTIC USE
In 90% of liver diseases there is a rise of γ-GT levels. The values are moderate in cirrhosis and infectious mononucleosis, while significant increases were found in obstructive liver disease, intrahepatic cholestasis or primary biliary cirrhosis.
Single test result could not be used to make a clinical diagnosis. It should integrate clinical and laboratory data.
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