LDL-CHOLESTEROL DIRECT, 1 x 80 ml

PRINCIPLE

The LDL-cholesterol reagent allows measuring the LDL-cholesterol fraction levels in serum or plasma directly, without the need for any sample pretreatment or centrifugation. The method consists of two specific steps. In the first one, chylomicron, VLDL and HDL are selectively eliminated by means of specific oxidation reactions with cholesterol esterase and cholesterol oxidase, and also by a specific degradation reaction with catalase, forming cholestenone and H2O2. In the second step, the remainder of LDL-cholesterol can be spectrophotometrically measured when a colour is formed as it is transformed (quinone complex) by the presence of specifi c surfactants.

DIAGNOSTIC USE

LDL are low density lipoprotein, they transport cholesterol, triglycerides and lipids to cells. Even within the normal range of total cholesterol concentrations, an increase in LDL cholesterol can occur with an associated increased risk for coronary heart disease. High levels of LDL could be associated with obesity, diabetes, and nephrosis. A single test result could not be used to make a clinical diagnosis. It should integrate clinical and laboratory data.