ADENOSINE DEAMINASE (ADA), 50 mL

PRINCIPLE

The hydrolysis of the adenosine present in the sample is catalyzed by adenosine deaminase (ADA), producing ammonium ions and inosine. Ammonium ions react with ?-Ketoglutarate by the action of glutamate dehydrogenase, oxidizing the NADPH to NADP .The Adenosine concentration present in the sample is proportional to the decrease in the concentration of NADPH in the reaction.

DIAGNOSTIC USE

ADA is one of the enzymes of purine metabolism present in microorganisms, plants and animals. The enzyme is widely distributed in human tissues, especially in lymphocytes. Elevated serum ADA activity has been observed in patients with liver diseases. Increased ADA activity in pleural fl uid was also observed in patients with tuberculosis eff usions.Single test result can not be used to make a clinical diagnosis. It should integrate clinical and laboratory data.