LIPASE, 80 mL

PRINCIPLE

At alkaline pH the lipase substrate 1,2-O-dilauryl-rac-glycero-3- glutaric acid-(6-methylresorufin)-esther is cleaved by the catalytic action of pancreatic lipase to form 1,2-O-dilauryl-rac-glycerol and unstable compound glutaric acid-(6-methylresorufi n)-esther. In that alkaline solution, this compound degrades to glutaric acid and methyl-resorufin. The color intensity of the red dye formed is directly proportional to the lipase activity. This activity can be measured at 578nm.

DIAGNOSTIC USE

The lipase is increased in pancreatitis from any source, and these values remain high longer than amylase values.

In chronic disorders of the pancreas the Lipase value is high and the same applies in cases of primary biliary cirrhosis, chronic renal failure and hemodialysis patients.

Single test result could not be used to make a clinical diagnosis. It should integrate clinical and laboratory data.